DDP1, Vigilin
Enables mRNA 3'-UTR binding activity; satellite DNA binding activity; and single-stranded DNA binding activity. Involved in chromosome condensation; chromosome segregation; and regulation of gene expression. Located in heterochromatin; nucleus; and polytene chromosome chromocenter. Part of ribonucleoprotein complex. Is expressed in several structures, including adult head; adult heart; anterior endoderm; embryonic/larval midgut primordium; and gut section. Used to study prostate cancer. Orthologous to human HDLBP (high density lipoprotein binding protein).
The gene Dodeca-satellite-binding protein 1 is referred to in FlyBase by the symbol Dmel\Dp1 (CG5170, FBgn0027835). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (1 unique). Gene sequence location is 2R:18412027..18421124. Its molecular function is described by: mRNA 3'-UTR binding; RNA binding; satellite DNA binding; mRNA binding; single-stranded DNA binding. It is involved in the biological process described with: chromosome condensation; heterochromatin formation; chromosome segregation; positive regulation of translation. 22 alleles are reported. The phenotypes of these alleles manifest in: mitotic M phase; anatomical structure; female organism; material anatomical entity; cell cycle phase. The phenotypic classes of alleles include: increased mortality; phenotype; increased mortality during development; abnormal cell number. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, at stages throughout the pupal period, in adult female stages.
Please see the JBrowse view of Dmel\Dp1 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.50
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.55
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dp1 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Dp1 protein binds dodeca-satellite DNA sequences with high affinity. Dp1 localizes to the heterochromatic chromocenter. It also localizes to euchromatic sites on all chromosomes, colocalizing with Su(var)205 protein.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Dp1 in JBrowseThe gene 'ToolKit' contains a set of key genetic reagents that can be used to study a gene. A single reagent for each category is chosen based on frequency of usage, and stock availability. Click "See all" to view all the reagents for the category.
Category | Common alleles (# stocks) |
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Classical and Insertion Alleles | |
Loss of function allele | |
Amorphic allele | |
Fluorescently-tagged allele | |
Transgenic Constructs | |
UAS RNAi | |
UAS wild-type cDNA | |
Untagged genomic rescue | |
Fluorescently-tagged genomic rescue | |
Aberrations | |
Deficiency | |
Duplication |
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Dp1 encodes a single stranded DNA-binding protein that binds the unstructured dodeca-satellite C-strand in pericentric heterochromatin with high affinity and specificity.
Source for merge of: Dp1 BcDNA:LD21677
Source for merge of: Dp1 anon-WO0118547.516 anon-WO0172774.168
Source for merge of: Dp1 BcDNA:LD21383
Dp1 complements a Scer\SCP160 deletion in yeast, a multi KH domain protein that is involved in control of cell ploidy.
Source for merge of Dp1 BcDNA:LD21677 was sequence comparison ( date:000202 ).
Source for merge of Dp1 anon-WO0118547.516 anon-WO0172774.168 was sequence comparison ( date:051113 ).
Source for merge of Dp1 BcDNA:LD21383 was sequence comparison ( date:000202 ).