p15
Involved in poly(A)+ mRNA export from nucleus. Located in cytoplasm; nuclear envelope; and nucleoplasm. Part of transcription repressor complex. Is expressed in several structures, including antennal primordium; anterior endoderm; embryonic/larval midgut primordium; gut section; and head epidermis primordium. Orthologous to human NXT1 (nuclear transport factor 2 like export factor 1) and NXT2 (nuclear transport factor 2 like export factor 2).
The gene NTF2-related export protein 1 is referred to in FlyBase by the symbol Dmel\Nxt1 (CG12752, FBgn0028411). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (all unique). Gene sequence location is 2R:23811321..23811974. Its molecular function is described by: protein binding. It is involved in the biological process described with: negative regulation of innate immune response; defense response to Gram-negative bacterium; poly(A)+ mRNA export from nucleus; nucleocytoplasmic transport. 16 alleles are reported. The phenotypes of these alleles manifest in: Z disc; mesonotum; sarcomere. The phenotypic classes of alleles include: lethal - all die during larval stage; partially lethal - majority die; visible; viable; some die during pupal stage; lethal - all die before end of pupal stage. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages.
Please see the JBrowse view of Dmel\Nxt1 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.52
Alternative translation stop created by use of multiphasic reading frames within coding region.
None of the polypeptides share 100% sequence identity.
Forms a complex with RanGAP, Nup358/RanBP2 and sbr/Nxf1 (PubMed:14729961). In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). The complex is knowns as Panx-induced cotranscriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). Interacts with nxf2 (via NTF2 domain); the interaction is direct and prevents Nxt1 binding to nucleoporins (PubMed:31368590). Interacts with sbr/nxf1 (PubMed:31570835). Interacts with Nup54 and Nup58 (PubMed:33856346).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nxt1 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as muscle system primordium
Comment: reported as muscle system primordium
JBrowse - Visual display of RNA-Seq signals
View Dmel\Nxt1 in JBrowseThe gene 'ToolKit' contains a set of key genetic reagents that can be used to study a gene. A single reagent for each category is chosen based on frequency of usage, and stock availability. Click "See all" to view all the reagents for the category.
Category | Common alleles (# stocks) |
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Classical and Insertion Alleles | |
Loss of function allele | |
Amorphic allele | |
Fluorescently-tagged allele | |
Transgenic Constructs | |
UAS RNAi | |
UAS wild-type cDNA | |
Untagged genomic rescue | |
Fluorescently-tagged genomic rescue | |
Aberrations | |
Deficiency | |
Duplication |
2-105
2-109.4
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Depletion of Nxt1 in SL2 cells (using RNAi) inhibits cell growth and results in a rapid and robust accumulation of polyadenylated RNAs within the nucleus.