FlyBase Gene Report: Dmel\Pis

General Information

Symbol

Dmel\Pis

Species

D. melanogaster

Name

Phosphatidylinositol synthase

Annotation Symbol

CG9245

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030670

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

CDP-diacylglycerol--inositol 3-phosphatidyltransferase (2.7.8.11)

Gene Summary

Phosphatidylinositol synthase (Pis) encodes an enzyme involved in the synthesis of phosphatidyl inositol, which is further modified to give rise to phosphorylated membrane lipids with multiple functions. It is important for membrane composition and Pis mutants develop various cellular polarity abnormalities, e.g. epithelial cells will misposition basement membrane proteins at their apical side, due to a lack of PIP2. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dPIS

Key Links

Genomic Location

Cytogenetic map

13E8-13E8

Sequence location

X:15,701,451..15,703,770 [-]

Recombination map

1-51

RefSeq locus

NC_004354 REGION:15701451..15703770

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

enzyme

regulator

receptor

receptor binding

transporter

structural molecule

cytoskeletal binding

DNA binding

RNA binding

transcription factor

small molecule binding

metal ion binding

lipid binding

carbohydrate binding

other molecular_function

molecular function

cell cycle/proliferation

cell organization/biogenesis

transport/localization

development

reproduction

immune system

nervous system process

behavior

response to stimulus

signaling

gene expression

protein metabolism

DNA metabolism

small molecule metabolism

other biological_process

biological process

extracellular region

cytosol

cytoskeleton

mitochondrion

nucleus

chromosome

membrane

cell periphery

cell junction

cell projection

endomembrane system

synapse

macromolecular complex

other cellular_component

cellular component

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity

inferred from direct assay

(Wang and Montell, 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000394714

(GO Reference Genome Project, 2011-)

enables phosphotransferase activity, for other substituted phosphate groups

inferred from electronic annotation with InterPro:IPR000462

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in basement membrane organization

inferred from mutant phenotype

(Devergne et al., 2014)

involved_in phototransduction

inferred from mutant phenotype

(Wang and Montell, 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in phosphatidylinositol biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000394714

(GO Reference Genome Project, 2011-)

involved_in phospholipid biosynthetic process

inferred from electronic annotation with InterPro:IPR000462

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN000394714

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR000462

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

OTHER SUBSTITUTED PHOSPHATE GROUP PHOSPHOTRANSFERASES

Protein Family (UniProt)

Belongs to the CDP-alcohol phosphatidyltransferase class-I family. (Q8SX37)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity

a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3- phospho-(1D-myo-inositol) + CMP + H(+) (2.7.8.11)

RHEA 11580:

Summaries

Gene Model and Products

Expression Data

Gene ToolKit

The gene 'ToolKit' contains a set of key genetic reagents that can be used to study a gene. A single reagent for each category is chosen based on frequency of usage, and stock availability. Click "See all" to view all the reagents for the category.

Category	Common alleles (# stocks)
Classical and Insertion Alleles
Loss of function allele
Amorphic allele
Fluorescently-tagged allele
Transgenic Constructs
UAS RNAi
UAS wild-type cDNA
Untagged genomic rescue
Fluorescently-tagged genomic rescue
Aberrations
Deficiency
Duplication

Alleles, Insertions, Transgenic Constructs, and Aberrations

Variants

Phenotypes

Orthologs

Paralogs

Human Disease Associations

Functional Complementation Data

Interactions

Pathways

Class of Gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-51

Cytogenetic map

13E8-13E8

Sequence location

X:15,701,451..15,703,770 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13E8-13E8

Limits computationally determined from genome sequence between P{EP}CG6340^EP1109&P{EP}l(1)G0168^EP491 and P{EP}EP1489

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-51

(FlyBase, 2016)

1-51.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Other Comments

Relationship to Other Genes

Nomenclature History

Synonyms and Secondary IDs (12)

Datasets (0)

External Crossreferences and Linkouts ( 73 )

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

GO Summary Ribbons

Expression Summary Ribbons

-0.79

Gene ToolKit

Disease Model Summary Ribbon

Report Sections