FlyBase Gene Report: Dmel\Plod

General Information

Symbol

Dmel\Plod

Species

D. melanogaster

Name

procollagen lysyl hydroxylase

Annotation Symbol

CG6199

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036147

Gene Model Status

Current

Stock Availability

11 publicly available

Enzyme Name (EC)

procollagen-lysine 5-dioxygenase (1.14.11.4)

procollagen galactosyltransferase (2.4.1.50)

procollagen glucosyltransferase (2.4.1.66)

Gene Summary

procollagen lysyl hydroxylase (Plod) encodes a lysyl dehydrogenase with roles in Collagen IV biosynthesis. It localizes to the endoplasmic reticulum and is required for the secretion Collagen IV from haemocytes and fat body. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dPlod

Key Links

Genomic Location

Cytogenetic map

68B1-68B1

Sequence location

3L:11,198,166..11,206,016 [-]

Recombination map

3-35

RefSeq locus

NT_037436 REGION:11198166..11206016

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

enzyme

regulator

receptor

receptor binding

transporter

structural molecule

cytoskeletal binding

DNA binding

RNA binding

transcription factor

small molecule binding

metal ion binding

lipid binding

carbohydrate binding

other molecular_function

molecular function

cell cycle/proliferation

cell organization/biogenesis

transport/localization

development

reproduction

immune system

nervous system process

behavior

response to stimulus

signaling

gene expression

protein metabolism

DNA metabolism

small molecule metabolism

other biological_process

biological process

extracellular region

cytosol

cytoskeleton

mitochondrion

nucleus

chromosome

membrane

cell periphery

cell junction

cell projection

endomembrane system

synapse

macromolecular complex

other cellular_component

cellular component

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables procollagen galactosyltransferase activity

inferred from mutant phenotype

(Bunt et al., 2011)

enables procollagen glucosyltransferase activity

inferred from mutant phenotype

(Bunt et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables iron ion binding

inferred from electronic annotation with InterPro:IPR006620

(InterPro Project Members, 2004-)

enables L-ascorbic acid binding

inferred from electronic annotation with InterPro:IPR006620

(InterPro Project Members, 2004-)

enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

inferred from electronic annotation with InterPro:IPR006620

(InterPro Project Members, 2004-)

enables procollagen-lysine 5-dioxygenase activity

inferred from sequence or structural similarity with UniProtKB:Q02809

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in growth of a germarium-derived egg chamber

inferred from mutant phenotype

(Lerner et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in collagen fibril organization

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in endoplasmic reticulum

inferred from direct assay

(Bunt et al., 2011)

located_in extracellular space

inferred from direct assay

(Bunt et al., 2011)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Bunt et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in collagen-containing extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

is_active_in endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN000842962

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

PEPTIDYL-LYSINE 5-DIOXYGENASES

PROCOLLAGEN GALACTOSYLTRANSFERASES

UNCLASSIFIED GLUCOSYLTRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

procollagen glucosyltransferase activity

(5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysyl-[collagen] + UDP-alpha-D- glucose = (5R)-5-O-[alpha-D-glucosyl-(1->2)-beta-D-galactosyl]-5-hydroxy- L-lysyl-[collagen] + UDP + H(+) (2.4.1.66)

RHEA 12576: procollagen-lysine 5-dioxygenase activity

L-lysyl-[collagen] + 2-oxoglutarate + O2 = (5R)-5-hydroxy-L-lysyl- [collagen] + succinate + CO2 (1.14.11.4)

RHEA 16569: procollagen galactosyltransferase activity

(5R)-5-hydroxy-L-lysyl-[collagen] + UDP-alpha-D-galactose = (5R)-5- O-(beta-D-galactosyl)-5-hydroxy-L-lysyl-[collagen] + UDP + H(+) (2.4.1.50)

RHEA 12637:

Summaries

Gene Model and Products

Expression Data

Gene ToolKit

The gene 'ToolKit' contains a set of key genetic reagents that can be used to study a gene. A single reagent for each category is chosen based on frequency of usage, and stock availability. Click "See all" to view all the reagents for the category.

Category	Common alleles (# stocks)
Classical and Insertion Alleles
Loss of function allele
Amorphic allele
Fluorescently-tagged allele
Transgenic Constructs
UAS RNAi
UAS wild-type cDNA
Untagged genomic rescue
Fluorescently-tagged genomic rescue
Aberrations
Deficiency
Duplication

Alleles, Insertions, Transgenic Constructs, and Aberrations

Variants

Phenotypes

Orthologs

Paralogs

Human Disease Associations

Functional Complementation Data

Interactions

Pathways

Class of Gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-35

Cytogenetic map

68B1-68B1

Sequence location

3L:11,198,166..11,206,016 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68B1-68B1

Limits computationally determined from genome sequence between P{PZ}l(3)01239⁰¹²³⁹&P{lacW}l(3)01239^j9B4 and P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-35

(FlyBase, 2016)

3-28.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Other Comments

Relationship to Other Genes

Nomenclature History

Synonyms and Secondary IDs (12)

Datasets (0)

External Crossreferences and Linkouts ( 50 )

References (64)

The following external sites may use different assemblies or annotations than FlyBase.

GO Summary Ribbons

Expression Summary Ribbons

-0.91

Gene ToolKit

Disease Model Summary Ribbon

Report Sections