Sulf1 is expressed in all provein regions in the wing imaginal disc.

Sulf1 is first detected in early first instar larvae in the central region of the wing pouch and in several spots located in the notum region of the disc. In the pouch, expression is further patterned during mid- to late- third instar stages. The domain in the central region of the wing pouch appears much broader. At late third instar larval stages, regionalization of the Sulf1 epxression domain occurs, defining stripes along the AP and DV axes reminiscent of provein domains L1 to L5. A lower level of staining is seen in the presumptive L3-L4 intervein region. Comparison to kn and dpp markers shows that expression is initially restricted to the anterior compartment but starting in mid-third instar larval stages extends to the posterior compartment. At the late third instar larval stage, Sulf1 is highly expressed in the L3 and L4 provein domains. In addition, expression is seen in L2 and L5 proveins and along the DV boundary.

Sulf1 is expressed at the morphogenetic furrow and in the central brain region in larvae. It is also expressed at high levels near the AP and DV compartment boundaries in wing discs.

Sulf1 displays weak and non-uniform staining in the lateral neurogenic ectoderm.