D69
Enables myosin binding activity and translation repressor activity. Involved in establishment of RNA localization; negative regulation of translation; and positive regulation of mRNA catabolic process. Located in P-body. Is expressed in several structures, including antennal primordium; central brain primordium; ganglia; germline cell; and head epidermis primordium. Orthologous to human SAMD4A (sterile alpha motif domain containing 4A) and SAMD4B (sterile alpha motif domain containing 4B).
The gene smaug is referred to in FlyBase by the symbol Dmel\smg (CG5263, FBgn0016070). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (2 unique). Gene sequence location is 3L:8990317..8997989. Its molecular function is described by 6 unique terms, many of which group under: binding; nucleic acid binding; RNA binding; mRNA binding; translation repressor activity. It is involved in the biological process described with 10 unique terms, many of which group under: cytoplasm organization; localization; nucleic acid biosynthetic process; macromolecule localization; oocyte construction. 31 alleles are reported. The phenotypes of these alleles manifest in: primordial germ cell; ovary; embryo; larval multidendritic class IV neuron. The phenotypic classes of alleles include: lethal; increased mortality during development; phenotype; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
RNA binding protein - repressor of Nanos translation - required for cleavage-independent maternal transcript destruction during the Drosophila MZT
Please see the JBrowse view of Dmel\smg for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Interacts with oskar (osk). Binds to the 3'-UTR of nanos (nos). Interacts with cup, which in turn recruits eIF4-E, leading to an indirect interaction between smg and eIF4-E that prevents mRNA translation. Forms a complex with aub, twin, AGO3, nanos mRNA and piRNAs that targets the nanos 3'-untranslated region, in early embryos.
The SAM domain mediates the association with the 3'-UTR of specific mRNAs.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\smg using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: rapidly degraded
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: labeled as "primordial germ cell"
smg protein levels as seen on western blots progressively increase through the early cleavage divisions, peak during syncytial blastoderm cycles 10-13, and then dramatically decline during interphase of cycle 14.
JBrowse - Visual display of RNA-Seq signals
View Dmel\smg in JBrowseThe gene 'ToolKit' contains a set of key genetic reagents that can be used to study a gene. A single reagent for each category is chosen based on frequency of usage, and stock availability. Click "See all" to view all the reagents for the category.
Category | Common alleles (# stocks) |
---|---|
Classical and Insertion Alleles | |
Loss of function allele | |
Amorphic allele | |
Fluorescently-tagged allele | |
Transgenic Constructs | |
UAS RNAi | |
UAS wild-type cDNA | |
Untagged genomic rescue | |
Fluorescently-tagged genomic rescue | |
Aberrations | |
Deficiency | |
Duplication |
3-28
3-21.3
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
smg is a major regulator of maternal mRNA destabilization.
Cloned by ability to arrest the cell cycle when expressed in the fission yeast S.pombe.
Source for merge of: smg anon-D69
Source for merge of smg anon-D69 was sequence comparison ( date:000202 ).